Journal: Scientific Reports

Article Title: Subfamily-selective PCR primers for the human LINE1 L1PA lineage

doi: 10.1038/s41598-025-17649-z

Figure Lengend Snippet: Quantification of L1PA subfamily RNA signals in human liver using subfamily-selective PCR primers. ( a ) LINE1 RT-qPCR analysis of human liver RNA using the indicated primers. Data are presented as mean RT-qPCR levels normalized to GAPDH ± standard deviation (s.d.), n = 3 technical replicates. ( b ) RNA-seq analysis of L1PA subfamilies in human liver using publicly available data. Summed FPKM values of annotated loci per L1PA subfamily are shown. Data are presented as mean ± s.d., n = 3 biological replicates. Data include multi-mapping reads; a corresponding analysis restricted to unique alignments is presented in Supplementary Fig. 3. ( c ) LINE1 RT-qPCR analysis of HEK293T cells following treatment with 5-azacytidine using the indicated primers. Data are presented as mean RT-qPCR levels normalized to GAPDH ± standard deviation (s.d.), n = 3 biological replicates. Statistical significance was assessed using a two-tailed t-test and *, ** and *** indicate p < 0.05, 0.01 and 0.001, respectively.

Article Snippet: cDNA synthesis was performed on human liver RNA (Amsbio R1234151-50) with SuperScript II Reverse Transcriptase (ThermoFisher).

Techniques: Quantitative RT-PCR, Standard Deviation, RNA Sequencing, Two Tailed Test

Journal: Biomedicines

Article Title: Developments and Applications of Liver-on-a-Chip Technology—Current Status and Future Prospects

doi: 10.3390/biomedicines13061272

Figure Lengend Snippet: Summary of select commercially available liver-on-a-chip platforms.

Article Snippet: Organovo (San Diego, CA, USA) , ExViveTM Human Liver Tissue , Bioprinted 3D liver constructs from primary human cells , No formal regulatory partnerships reported , Applied in preclinical DILI and fibrosis studies.

Techniques: Imaging, Construct, Drug discovery